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Image Search Results
Journal: Cancers
Article Title: C3G Is Upregulated in Hepatocarcinoma, Contributing to Tumor Growth and Progression and to HGF/MET Pathway Activation
doi: 10.3390/cancers12082282
Figure Lengend Snippet: C3G knock-down reduces in vitro tumorigenic properties of HCC cells. Hep3B and HLE non-silenced (−) and C3G knock-down (shC3G) cells; and mHCC1 (non-silenced (−) and C3G knock-down (clones 2.17, 1.2 and 1.3)) were maintained in complete medium. ( A , B ) Top panels: Western-blot analysis of C3G expression in HCC cells, normalized with β-actin or tubulin. Middle panels: Representative macroscopic (whole dish) and microscopic (individual foci) photographs of anchorage-dependent growth assay. Bottom panels: Histograms show the mean ± S.E.M. of the number of foci (%) and the number of cells per focus (%). ( C ) Upper panels: Western-blot analysis of C3G expression in mHCC1 cells, normalized with β-actin. Middle panels: Representative macroscopic (whole dish) and microscopic (individual foci) photographs of anchorage-dependent growth assay. Lower panel: Histogram shows the mean ± S.E.M. of the number of foci. ( D ) Anchorage independent growth of Hep3B cells. Left panel: Histograms represent the mean ± S.E.M. of the number of foci (left) and cells per focus (right). Right panels: Representative microscope images of foci formation. ( E ) Adhesion assay in Hep3B cells. Histogram represents the mean ± S.E.M. of the percentage of adhered cells (%) after 15 and 30 min. Individual cells were counted with ImageJ. ( F ) Histograms showing the mean ± S.E.M. of the percentage of Hep3B cells in S+G2/M phases or ( G ) with a lower DNA content than 2C (apoptotic) analyzed by cytometry, when maintained in culture with 10% serum under adherent or suspension conditions for 6h. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, vs. non-silenced cells (−), ( A , B , D , E ) analyzed by Student’s t -test, ( A , B ) n > 3 or ( C , F , G ) one-way ANOVA; n ≥ 3.
Article Snippet: Then, membranes were probed with primary antibodies listed in CTAT table. β-Actin (Cell Signalling, Leiden, The Netherlands, #3700) or
Techniques: Knockdown, In Vitro, Clone Assay, Western Blot, Expressing, Growth Assay, Microscopy, Cell Adhesion Assay, Cytometry, Suspension